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New Zealand Beekeeping Forums
New Zealand Beekeeping Disease & Pests
NZBeekeeping has launched a consultation for the AFB PMP 10 year review
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<blockquote data-quote="JohnF" data-source="post: 10566" data-attributes="member: 233"><p>I'm not telling you anything new [USER=149]@Morporks[/USER] - but by the time AFB is roping, its spread already. And yes, we are racing to get our new work published showing that high levels of AFB (vegetative and spores) detected at the entrance, correlates extremely well with clinical signs present - or within a short time in the future.</p><p>If we detect low levels of spores then this is not an issue - it is only when we reach a threshold of thousands of spores. It is possible to use PCR in a format to detect viable vs non-viable but this is more time-consuming (ie expensive). However, we can use the quantitative nature of the method to know that if we detect high levels of the pathogen then most of this will certainly be viable. </p><p>DNA is a food source (carbon) for other microbes and bacteria and - like varroa on a sticky board - don't always last long free in the environment</p></blockquote><p></p>
[QUOTE="JohnF, post: 10566, member: 233"] I'm not telling you anything new [USER=149]@Morporks[/USER] - but by the time AFB is roping, its spread already. And yes, we are racing to get our new work published showing that high levels of AFB (vegetative and spores) detected at the entrance, correlates extremely well with clinical signs present - or within a short time in the future. If we detect low levels of spores then this is not an issue - it is only when we reach a threshold of thousands of spores. It is possible to use PCR in a format to detect viable vs non-viable but this is more time-consuming (ie expensive). However, we can use the quantitative nature of the method to know that if we detect high levels of the pathogen then most of this will certainly be viable. DNA is a food source (carbon) for other microbes and bacteria and - like varroa on a sticky board - don't always last long free in the environment [/QUOTE]
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New Zealand Beekeeping Forums
New Zealand Beekeeping Disease & Pests
NZBeekeeping has launched a consultation for the AFB PMP 10 year review
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