When a queen suddenly disappears, the colony can make a replacement for her. From any larva up to 3 days old. This is the biology of the bee. Plus the egg stage, before it turns into a larva. In total, the colony has 6 days to save itself. If during this period you will harm a new queen, she will be killed with a very high probability. Bees are much closer and more native to what they grow themselves, and not your purchased queen. Therefore, you had to calculate 7 days from the loss of your queen and review all the cells for the presence of queen cells. Sometimes you have to shake off the bees completely, so as not to miss them. you pull out the queen cells, or break them with a beekeeping chisel - the main thing is to kill the larva inside the queen cell. Now the colony has no chance to raise its queen. They feel orphaned, and are ready to accept a new queen. you put a cage with a new queen in the hive and wait a couple of days. the queen will calm down and the bees will feed it. before the release, it is better to check again for queen cells and virgin queens (the latter usually walk on the extreme limits) . And now we are releasing the queen. carefully. that would not take off. A good way is to take a blade of grass, dip it in honey from the honeycomb, and anoint the queen a little. The bee will lick it and transfer the pheromone faster. a good sign is that bees are arranged around the queen in the form of the sun. carefully place the frame back into the hive, and after 5-7 minutes check the condition of the queen again. If the behavior has not changed, then the queen has been accepted.
